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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Characterization of the complete chloroplast genome of Lonicera macranthoides
Huan Hu1, Jianguo Liu1, Jiaxing An2
1Special Key Laboratory of Microbial Resources and Drug Development from Higher Education Institution of Guizhou Province, Research Center for Medicine and Biology, Zunyi Medical University, Zunyi, China.
Abstract:
Lonicera macranthoides is an important Chinese traditional medicine plant which is endemic in southwest China. The complete chloroplast genome from L. macranthoides is determined in this study. The genome size was 154,897 bp, containing a large single copy (LSC) region of 88,692 bp and a small single copy (SSC) region of 18,623 bp, which were separated by a pair of 23,786 bp inverted repeat (IR) regions. The plastome contained 130 genes, including 81 protein-coding genes (77 PCG species), eight ribosomal RNA genes (4 rRNA species), 39 tRNA genes (29 tRNA species) and two pseudognes. The most of gene species occurred as a single copy, while 13 gene species occurred in double copies, including all rRNA species, six tRNA species and three PCG species, and two tRNA genes occur in treble copies. Finally, phylogenetic analysis demonstrated that L. macranthoides is closely related to L. japonica, and genus Lonicera shows a closer relationship with Triostrum for the current data.
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