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Renal MRI Diffusion: Experimental Protocol.
João S Periquito1, Martin Meier2, Thoralf Niendorf3
1Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Berlin, Germany. joao.periquito@mdc-berlin.de.
Methods in Molecular Biology (Clifton, N.J.)
|January 21, 2021
Summary
This study details a step-by-step protocol for measuring renal diffusion coefficients in rodents using diffusion-weighted imaging (DWI) with apparent diffusion coefficient (ADC) or intravoxel incoherent motion (IVIM) models. These advanced MRI techniques offer insights into kidney microstructure and potential biomarkers for renal function.
Area of Science:
- Medical Imaging
- Biophysics
- Nephrology
Background:
- Renal diffusion-weighted imaging (DWI) offers insights into kidney microstructure.
- It holds potential for developing MR-biomarkers for functional renal imaging.
- Understanding renal microstructure aids in comprehending physiology and early pathological processes.
Purpose of the Study:
- To provide a detailed experimental protocol for measuring renal diffusion coefficients in rodents.
- To describe the application of apparent diffusion coefficient (ADC) and intravoxel incoherent motion (IVIM) models in renal DWI.
- To highlight the utility of DWI in assessing renal microstructure.
Main Methods:
- Step-by-step experimental protocol for renal diffusion-weighted imaging (DWI) in rodents.
- Utilized both apparent diffusion coefficient (ADC) and intravoxel incoherent motion (IVIM) models for quantification.
- IVIM model allows calculation of pseudodiffusion and fraction from vascular and tubular components.
Main Results:
- Established a standardized MRI method for quantifying renal diffusion coefficients.
- Demonstrated the application of ADC and IVIM models for detailed microstructural analysis.
- IVIM model provides additional parameters related to tissue vascular and tubular contributions.
Conclusions:
- Renal DWI provides valuable information on kidney microstructure.
- ADC and IVIM models enable robust quantification of diffusion coefficients.
- This protocol contributes to the standardization of renal MRI biomarkers for improved reproducibility.