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Related Experiment Videos

Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST) MRI: Experimental Protocol.

Kowsalya Devi Pavuluri1, Lorena Consolino2, Dario Livio Longo3

  • 1Division of MR Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 21, 2021
PubMed
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Chemical exchange saturation transfer (CEST) MRI provides pH and perfusion maps for monitoring kidney health in rodents. This method aids in understanding kidney disease progression using novel imaging protocols.

Area of Science:

  • Biomedical Imaging
  • Magnetic Resonance Imaging
  • Renal Physiology

Background:

  • Kidney diseases pose a significant health burden, necessitating advanced diagnostic biomarkers.
  • pH homeostasis is a critical indicator of kidney function, making it a key target for monitoring.
  • Chemical exchange saturation transfer (CEST) MRI is a promising technique for non-invasive pH measurement.

Purpose of the Study:

  • To detail step-by-step Chemical Exchange Saturation Transfer (CEST) MRI protocols for rodent models.
  • To establish methods for generating pH and perfusion maps to assess kidney pH homeostasis.
  • To evaluate the impact of acquisition time on map quality for improved in vivo renal imaging.

Main Methods:

  • Utilized Chemical Exchange Saturation Transfer (CEST) Magnetic Resonance Imaging (MRI) in rodent models.
Keywords:
Chemical exchange saturation transfer (CEST)Contrast agentIopamidolKidneyMagnetic resonance imaging (MRI)MiceRatsResponsive contrast agentpH imaging

Related Experiment Videos

  • Administered iopamidol as a contrast agent to enhance imaging.
  • Developed and described various CEST techniques, acquisition protocols, and ratiometric approaches.
  • Assessed the influence of acquisition duration on the resultant pH and perfusion maps.

    • Main Results:

    • Successfully generated pH and perfusion maps of rodent kidneys.
    • Demonstrated the feasibility of monitoring kidney pH homeostasis using the described CEST MRI protocols.
    • Detailed the relationship between acquisition time and the quality of generated renal maps.

    Conclusions:

    • The described CEST MRI protocols offer a valuable tool for in vivo monitoring of kidney pH homeostasis in rodents.
    • These methods can aid in investigating the progression of kidney diseases in experimental models.
    • The work contributes to the standardization of renal MRI biomarkers, aligning with the goals of the COST Action PARENCHIMA.