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Updated: Nov 20, 2025

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
A novel sampling cassette for field-based analysis of respirable crystalline silica
Lauren G Chubb1, Emanuele G Cauda1
1Pittsburgh Mining Research Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Pittsburgh, Pennsylvania.
Abstract:
Field-based methods for the analysis of respirable crystalline silica are now possible with the availability of portable instrumentation. Such methods also require the use of cassettes that facilitate direct-on-filter analysis of field samples. Conventional sampling cassettes can be modified such that they are amenable to direct-on-filter analysis while remaining compatible with common respirable dust samplers. The required modifications are described herein, and one version of such an analysis-ready cassette is described and evaluated in comparison to more traditional cassette designs. The novel cassette was found to result in a slightly higher mass of collected respirable material (for the same sampling duration), though this is likely due to the conductive material of the cassettes, which prevents particle wall losses in comparison to the more commonly used styrene cassette material. Both types of cassettes demonstrated comparable predictability in terms of respirable crystalline silica in a sample.
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