Related Experiment Video

Updated: Nov 20, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Published on: August 20, 2021

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Complete Genome Sequence of Klebsiella pneumoniae Myophage Muenster

Cody Martin1, Lauren Lessor1, James Clark1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|January 22, 2021
PubMed

Insights

We sequenced the Klebsiella jumbo phage Muenster genome. Its genetic makeup, including tail fibers, may help it infect a wider range of antibiotic-resistant bacteria.

Area of Science:

  • Microbiology
  • Genomics
  • Virology

Background:

  • Klebsiella pneumoniae contributes to antibiotic-resistant hospital infections.
  • Bacteriophages are viruses that infect bacteria and are potential therapeutic agents.

Purpose of the Study:

  • To present the annotated genome sequence of the Klebsiella jumbo phage Muenster.
  • To identify genetic features that may influence the phage's host range.

Main Methods:

  • Whole-genome sequencing of the Klebsiella jumbo phage Muenster.
  • Bioinformatic analysis to annotate protein-coding genes and identify functional elements.

Main Results:

  • The Muenster genome is 346,937 base pairs long.
  • The genome encodes 6 tRNAs and 561 putative protein-coding genes.
  • Nine tail fiber genes were identified, suggesting a mechanism for host range expansion.

Conclusions:

  • The annotated genome provides a foundation for further research into Klebsiella phage biology.
  • The identified tail fiber genes offer insights into potential host-targeting strategies for phage therapy against Klebsiella pneumoniae.

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