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Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Related Experiment Video

Updated: Nov 20, 2025

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

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Exploring Phosphoinositide Binding Using Native Mass Spectrometry.

Julian Bender1, Carla Schmidt2

  • 1Interdisciplinary research center HALOmem, Institute for Biochemistry and Biotechnology, Charles Tanford Protein Centre, Martin Luther University Halle-Wittenberg, Halle, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|January 22, 2021
PubMed
Summary
This summary is machine-generated.

Native mass spectrometry (MS) enables studying how phosphoinositides interact with membrane proteins. This protocol details using MS and a biochemical assay to analyze these crucial cellular interactions.

Keywords:
Membrane proteinsNative mass spectrometryPhosphoinositidesProtein–phosphoinositide interactions

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Phosphoinositides are key regulators of cellular processes, often by recruiting proteins to membranes.
  • Understanding protein-phosphoinositide interactions is vital for deciphering cellular signaling and function.
  • Integral and peripheral membrane proteins play critical roles in cellular localization and activity.

Purpose of the Study:

  • To present a protocol for analyzing protein-phosphoinositide interactions using native mass spectrometry (MS).
  • To detail the application of native MS for both integral and peripheral membrane proteins.
  • To provide guidance on experimental setup, data analysis, and validation using a biochemical assay.

Main Methods:

  • Native mass spectrometry (MS) to preserve noncovalent protein-lipid interactions in the gas phase.
  • Application of native MS to integral and peripheral membrane proteins.
  • Development and description of a complementary biochemical assay for interaction validation.

Main Results:

  • Demonstration of native MS as a suitable technique for studying protein-phosphoinositide interactions.
  • Detailed workflow covering sample preparation, instrumental setup, and data analysis for native MS experiments.
  • Successful application of the protocol to both integral and peripheral membrane proteins.

Conclusions:

  • Native MS is a powerful tool for investigating the molecular mechanisms of phosphoinositide-protein binding.
  • The described protocol provides a comprehensive guide for researchers studying membrane protein-lipid interactions.
  • Integration of native MS with biochemical assays offers robust validation of observed interactions.