Related Experiment Video
Updated: Nov 20, 2025
10:27
Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
10.3K
Functionalization using polymer or silane? A practical test method to characterize hydrophilic interaction
Tohru Ikegami1, Ashin Taniguchi1, Tomoyuki Okada2
1Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Journal of Chromatography. A
|January 22, 2021
Summary
This study classifies 45 hydrophilic interaction chromatography (HILIC) columns into silane-modified and polymer-modified groups based on their selectivity. This classification aids in understanding HILIC column separation characteristics for improved method development.
Area of Science:
- Analytical Chemistry
- Chromatography
- Separation Science
Background:
- Hydrophilic interaction chromatography (HILIC) is crucial for separating polar small molecules.
- Distinguishing structural differences in complex matrices requires selective HILIC columns.
- Characterization of commercially available HILIC columns is needed for informed selection.
Purpose of the Study:
- To characterize commercially available HILIC columns for their selectivity.
- To classify HILIC columns based on their chemical properties and separation behavior.
- To provide a framework for improved HILIC column selection in complex analyses.
Main Methods:
- Characterization of 45 commercially available HILIC columns.
- Application of Principal Component Analysis (PCA) and cluster analysis.
- Determination of column selectivity for functional groups (e.g., OH, CH2) and ion-exchange properties.
Main Results:
- HILIC columns were classified into two main groups: silane-modified and polymer-modified.
- Further subdivisions were based on specific functionalities and surface properties.
- Column selectivity for OH groups and cation-exchange nature significantly impacted separations.
Conclusions:
- A systematic method for classifying HILIC columns based on selectivity was established.
- The classification provides insights into the separation characteristics of different HILIC columns.
- This study facilitates better selection of HILIC columns for specific analytical challenges.
Related Concept Videos
01:11High-Performance Liquid Chromatography: Introduction
2.9K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.9K
01:10Silica Gel Column Chromatography: Overview
2.6K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
2.6K
01:17Gas Chromatography: Types of Columns and Stationary Phases
1.7K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
1.7K
01:17Ion Exchange
844
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
844
01:09Analyte Adsorption and Distribution
1.8K
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
1.8K
02:08Introduction to Functional Groups
33.4K
Functional groups are group of atoms with specific chemical properties that occur within organic molecules and sometimes denoted as “R”. Functional groups are found along the carbon backbone of macromolecules can form chains or rings of carbon atoms. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
Types of common functional groups
The table below summarizes some of the major functional groups in organic chemistry....
33.4K