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Related Experiment Video

Updated: Nov 20, 2025

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Mouse Quantitative Proteomics Knowledgebase: reference protein concentration ranges in 20 mouse tissues using 5000

Yassene Mohammed1,2, Pallab Bhowmick1, Sarah A Michaud1

  • 1University of Victoria - Genome British Columbia Proteomics Centre, University of Victoria, Victoria, BC V8Z 7X8, Canada.

Bioinformatics (Oxford, England)
|January 23, 2021
PubMed
Summary

MouseQuaPro is a new knowledgebase detailing absolute protein concentrations in over 700 mouse tissue samples. This resource maps protein levels across tissues, strains, and sexes, aiding proteomics research.

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Last Updated: Nov 20, 2025

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Area of Science:

  • Proteomics
  • Systems Biology
  • Biomedical Research

Background:

  • Laboratory mice are crucial animal models in biological research due to conserved synteny with humans.
  • Mice are used to study gene function, disease pathology, and drug metabolism.
  • Quantitative proteomics data is essential for understanding biological systems.

Purpose of the Study:

  • To introduce MouseQuaPro, a comprehensive knowledgebase of quantitative proteomics data from mouse tissues.
  • To provide experimentally determined protein concentration ranges for over 1600 proteins.
  • To facilitate the design of proteomics phenotyping experiments.

Main Methods:

  • Development of over 5000 quantitative targeted proteomics assays for 20 mouse tissues.
  • Determination of protein concentration ranges using heavy labeled internal standards.
  • Creation of a web-based application integrating proteomics data with biological annotations.

Main Results:

  • MouseQuaPro hosts over 200,000 experimentally determined protein concentrations from >700 mouse tissue samples across three strains.
  • The knowledgebase integrates protein concentrations with human orthologs, pathways, diseases, and gene expression data.
  • Protein concentration ranges reveal similarities and differences between tissues, strains, and sexes.

Conclusions:

  • MouseQuaPro offers an initial map of absolute protein concentrations in mouse tissues.
  • The resource enables advanced search, filtering, and interactive visualization of proteomics data.
  • MouseQuaPro supports guided design of proteomics phenotyping experiments and comparative analyses.