Chemometrics-assisted simultaneous voltammetric determination of multiple neurotransmitters in human serum

An electrochemical method combining chemometrics was developed for simultaneous quantification of multiple neurotransmitters including Dopamine (DA), Epinephrine (EP), Norepinephrine (NE) and serotonin (5-hydroxytryptamine, 5-HT) in human blood serum. A reduced graphene oxide modified glassy carbon electrode (RGO/GCE) was prepared via electrodeposition method. Differential pulse voltammetry (DPV) measurement of the four neurotransmitters showed that the voltammetric signals of the four targets overlapped significantly. To facilitate the simultaneous determination of the neurotransmitters, a chemometric tool of Tchebichef curve moment (TcM) method was proposed. The TcMs calculated from the voltammograms were used to establish the quantitative models by stepwise regression. The intra-day and inter-day precisions of the proposed method were less than 3.5% and 8.1%, respectively, and the recoveries were from 87.4% to 124%. The limit of detection (LOD) for DA, EP, NE and 5-HT were 74 nM, 104 nM, 84 nM and 97 nM, respectively. The above results indicated that the proposed approach is simple and reliable for the simultaneous determination of multiple neurotransmitters in human serum.