Related Experiment Video
Updated: Nov 20, 2025

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
Published on: April 13, 2016
Multi-mode Sample Introduction System (MSIS) as an interface in the hyphenated system 2 HPLC-MSIS-ICP-OES in
Jędrzej Proch1, Przemysław Niedzielski1
1Adam Mickiewicz University, Faculty of Chemistry, Department of Analytical Chemistry, Uniwersytetu Poznańskiego 8, 61-614, Poznań, Poland.
Abstract:
The paper presents a usage of a new hyphenated technique, wherein a Multi-mode Sample Introduction System (MSIS) was applied as an interface of two high pressure liquid chromatography units and inductively coupled plasma optical emission spectrometry (2 HPLC-MSIS-ICP-OES). Simultaneous separation and detection of non-hydride forming and hydride forming elements was possible due to the application of two different HPLC column, cation-exchange and anion-exchange respectively. The method was able to determine 15 elements quantitatively with a distinction of three arsenic and two iron species and it was validated obtaining acceptable LODs (2.67-28.7 μg L-1) and recoveries (80-120%). The method applicability was presented and confirmed on 5 varied sample matrix types i.e. post-glacial sediments, yerba mate (Ilex paraguariensis), soil samples located in the proximity of industry wastes disposal site, river sediments, and archaeological pottery. In addition to the above, unknown Cu, Fe, Mn and Zn species were detected in real samples (qualitative speciation analysis) and the identification was attempted according to the literature.
Related Concept Videos
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Tandem Mass Spectrometry
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
High-Performance Liquid Chromatography: Elution Process
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....

