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Related Experiment Video

Updated: Nov 20, 2025

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

Functionally Active Eosinophil Purification from Peripheral Blood.

Kiho Son¹, Paige Lacy², Manali Mukherjee³

¹Department of Medicine, McMaster University & St Joseph's Healthcare, Hamilton, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|January 24, 2021

Summary

This summary is machine-generated.

This study introduces a faster method for isolating human peripheral blood eosinophils, crucial for accurate in vitro research. The MACSxpress® kit offers improved efficiency and purity for eosinophil analysis.

Keywords:

Cytospin Degranulation Eosinophils Flow cytometry Immunomagnetic Isolation Negative selection Population purity

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Last Updated: Nov 20, 2025

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Area of Science:

Immunology
Cell Biology

Background:

Eosinophil isolation is critical for in vitro research and clinical data interpretation.
Conventional methods involve density gradient centrifugation and immunomagnetic selection, which are time-consuming.
Advancements offer faster, more efficient isolation techniques with high purity.

Purpose of the Study:

To describe and evaluate a modern isolation procedure for human peripheral blood eosinophils.
To present protocols for assessing the purity and activation state of isolated eosinophils.
To demonstrate a functional assay for verifying eosinophil integrity.

Main Methods:

Utilized the MACSxpress® Whole Blood Isolation Kit for eosinophil isolation.
Employed differential staining and flow cytometry for purity and activation analysis.
Performed an in vitro degranulation assay to assess cell functionality.

Main Results:

The MACSxpress® method provides a significantly reduced processing time compared to conventional techniques.
High purity of isolated eosinophil populations was achieved.
Isolated eosinophils demonstrated intact functionality in degranulation assays.

Conclusions:

The MACSxpress® Whole Blood Isolation Kit offers an efficient and reliable method for obtaining pure, functional human eosinophils.
This technique facilitates more accessible and accurate in vitro immunological studies.
The described protocols support comprehensive analysis of eosinophil function and activation state.