Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors

Human eosinophilic leukocytes are found in peripheral blood and tissues at homeostasis and at elevated levels in atopic disorders. As inbred strains of mice (Mus musculus) are currently the models of choice for the study of disease mechanisms in vivo, a full understanding of mouse eosinophils is critical for interpretation of experimental findings. Toward this end, several years ago we presented a protocol for generating mouse eosinophils in tissue culture from unselected bone marrow progenitors (Dyer et al., J Immunol 181: 4004-4009, 2008). This method has been implemented widely and has proven to be effective for generating phenotypically normal eosinophils from numerous mouse strains and genotypes. Here we provide a detailed version of this protocol, along with suggestions and notes for its careful execution. We have also included several protocol variations and suggestions for improvements.