Related Experiment Video

Updated: Nov 20, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.9K

MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

Li Cai1, Lu Ye2, Xiaoqing Hu1

  • 1Department of Oncology, Jiangxi Maternal and Child Health Hospital , Nanchang, Jiangxi, China.

Bioengineered
|January 25, 2021
PubMed

Insights

MicroRNA-330-3p (miR-330-3p) is downregulated in ovarian cancer (OC), inhibiting its progression. Restoring miR-330-3p levels suppresses OC cell proliferation, migration, and invasion by targeting RIPK4.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-330-3p (miR-330-3p) has been implicated in various cancers, but its role in ovarian cancer (OC) remains largely unexplored.
  • Understanding the specific functions of miR-330-3p in OC is crucial for identifying potential therapeutic targets.

Purpose of the Study:

  • To investigate the expression profile and functional significance of miR-330-3p in ovarian cancer.
  • To elucidate the molecular mechanisms underlying miR-330-3p's action in OC progression.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (RT-qPCR) for miR-330-3p expression analysis in OC tissues and cell lines.
  • Cell Counting Kit-8 (CCK-8) and Transwell assays to assess OC cell proliferation, migration, and invasion.
  • Bioinformatics analysis, dual-luciferase reporter assays, and RNA immunoprecipitation (RIP) assays to identify and validate miR-330-3p targets, specifically RIPK4.

Main Results:

  • miR-330-3p was significantly downregulated in OC tissues and cell lines compared to normal controls.
  • Overexpression of miR-330-3p markedly inhibited the proliferation, migration, and invasion capabilities of OC cells.
  • RIPK4 was identified as a direct target gene of miR-330-3p, and miR-330-3p suppressed OC progression by regulating RIPK4.

Conclusions:

  • miR-330-3p acts as a tumor suppressor in ovarian cancer by inhibiting cell proliferation, migration, and invasion.
  • The miR-330-3p/RIPK4 axis represents a promising novel therapeutic target for ovarian cancer treatment.
Keywords:
Mir-330-3pRIPK4ovarian cancer

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.8K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.6K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.2K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.8K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.1K