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Long non-coding RNA ADAMTS9-AS1 attenuates ferroptosis by Targeting microRNA-587/solute carrier family 7 member 11

Li Cai1, Xiaoqing Hu1, Lu Ye2

  • 1Department of gynecologic oncology, Maternal and Child Health Affiliated Hospital of Nanchang University Nanchang 330006, Jiangxi, China.

Bioengineered
|March 21, 2022
PubMed

Insights

Long non-coding RNA ADAMTS9-AS1 inhibits ferroptosis in epithelial ovarian cancer (EOC) by targeting the microRNA-587/solute carrier family 7 member 11 axis, offering a potential therapeutic target for EOC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Epithelial ovarian cancer (EOC) is the leading cause of gynecological cancer mortality.
  • Understanding EOC molecular mechanisms is crucial for improved diagnostics and treatments.

Purpose of the Study:

  • To investigate the role of long non-coding RNA ADAMTS9-AS1 (ADAMTS9-AS1) in regulating solute carrier family 7 member 11 (SLC7A11) expression and ferroptosis in EOC.
  • To elucidate the mechanism involving microRNA-587 (miR-587) sponging by ADAMTS9-AS1.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting to assess gene expression.
  • Cell function assays and ferroptosis marker detection.
  • Dual-luciferase reporter gene and RNA immunoprecipitation assays to confirm molecular interactions.

Main Results:

  • ADAMTS9-AS1 expression was elevated in EOC cells.
  • Knocking down ADAMTS9-AS1 increased miR-587 and decreased SLC7A11 expression, inhibiting proliferation and migration by promoting ferroptosis.
  • ADAMTS9-AS1 directly sponged miR-587, which in turn targeted SLC7A11.

Conclusions:

  • ADAMTS9-AS1 attenuates ferroptosis in EOC by targeting the miR-587/SLC7A11 axis.
  • ADAMTS9-AS1 represents a potential therapeutic target for epithelial ovarian cancer.
Keywords:
Long non-coding RNA ADAMTS9-AS1epithelial ovarian cancerferroptosismicoRNA-587solute carrier family 7 member 11

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