Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

654
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
654
CRISPR01:59

CRISPR

55.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
55.0K
What is Genetic Engineering?00:49

What is Genetic Engineering?

77.3K
Overview
77.3K
Genetic Screens02:46

Genetic Screens

5.3K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.3K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improvement in Th1/Th2 balance and endometrial receptivity after hysteroscopic surgery in patients with cesarean scar disorder: a case report.

Maternal health, neonatology and perinatology·2026
Same author

"Shortening time to pregnancy in infertile women by personalizing treatment of microbial imbalance through Emma & Alice: A multicenter prospective study".

Reproductive medicine and biology·2025
Same author

Effects of first and second division modes on euploidy acquisition in human embryo.

Systems biology in reproductive medicine·2024
Same author

Smooth endoplasmic reticulum cluster presence does not affect embryo ploidy.

Archives of gynecology and obstetrics·2023
Same author

The effects of differences in trophectoderm biopsy techniques and the number of cells collected for biopsy on next-generation sequencing results.

Reproductive medicine and biology·2022
Same author

A novel trophectoderm biopsy technique for all blastocyst stages.

Reproductive medicine and biology·2022

Related Experiment Video

Updated: Nov 20, 2025

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.3K

Pre-implantation genetic testing: Past, present, future.

Kazuhiro Takeuchi1

  • 1Takeuchi Ladies Clinic/Center for Reproductive Medicine Aira-shi Japan.

Reproductive Medicine and Biology
|January 25, 2021
PubMed
Summary

Pre-implantation genetic testing (PGT) has evolved from FISH to NGS. Blastocyst biopsy with NGS offers more reliable diagnoses, leading to higher pregnancy and lower abortion rates in PGT-SR.

Keywords:
PGTTE biopsyfluorescencein situ hybridizationnext‐generation sequencing

More Related Videos

FISH for Pre-implantation Genetic Diagnosis
07:34

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

37.5K
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

9.7K

Related Experiment Videos

Last Updated: Nov 20, 2025

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

3.3K
FISH for Pre-implantation Genetic Diagnosis
07:34

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

37.5K
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

9.7K

Area of Science:

  • Reproductive Medicine
  • Genetics
  • Embryology

Background:

  • Pre-implantation genetic testing (PGT) has evolved significantly since 1990.
  • Early PGT utilized cleavage stage biopsy and FISH, transitioning to blastocyst biopsy (TE biopsy) and NGS around 2012.
  • NGS has become the current worldwide standard for PGT analysis.

Purpose of the Study:

  • To compare the clinical outcomes of FISH and NGS in PGT for structural rearrangements.
  • To evaluate the effectiveness of TE biopsy and NGS for PGT-SR in Japan.

Main Methods:

  • PGT for reciprocal balanced translocation and Robertsonian translocation (PGT-SR) was performed using cleavage stage embryos and FISH since 2008.
  • In 2014, the study transitioned to trophectoderm (TE) biopsy and next-generation sequencing (NGS) analysis.
  • Detailed methods and clinical results for both FISH and NGS were described.

Main Results:

  • NGS demonstrates superiority over FISH due to its ability to detect all chromosomes.
  • TE biopsy yields more cells and fewer mosaic results compared to cleavage stage biopsy.
  • This leads to more reliable diagnoses.

Conclusions:

  • Trophectoderm (TE) biopsy combined with NGS provides stable outcomes for PGT.
  • The improved diagnostic reliability of TE biopsy and NGS results in higher pregnancy rates.
  • Consequently, a reduction in abortion rates is observed with the newer PGT methods.