Isolation of tumor-resident CD8⁺ T cells from human lung tumors

CD103⁺CD8⁺ tumor-resident memory T cells (T_RM) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8⁺ T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8⁺ T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of T_RM cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).