Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

4.1K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
4.1K
Deconvolution01:20

Deconvolution

414
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
414
Uniform Depth Channel Flow01:27

Uniform Depth Channel Flow

342
Uniform depth channel flow keeps fluid depth consistent along channels such as irrigation canals. In natural channels, such as rivers, approximate uniform flow is often assumed. This condition occurs when the channel’s bottom slope matches the energy slope, balancing potential energy lost from gravity with head loss due to shear stress. This balance prevents depth changes along the channel length, resulting in a steady, uniform flow.Uniform flow in open channels with a constant cross-section...
342
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.9K
Shape and Texture of Coarse Aggregate01:25

Shape and Texture of Coarse Aggregate

369
Aggregate shape is classified based on the relative sharpness or roundness of the edges and corners. This classification includes categories like rounded, angular, elongated, and flaky, each with specific characteristics. Rounded aggregates, fully shaped by attrition, are typical of river or seashore gravel, while angular aggregates, such as crushed rock, have well-defined edges. Aggregates that are elongated and flaky are less desirable, as they can reduce the workability and strength of...
369
Uniform Depth Channel Flow: Problem Solving01:18

Uniform Depth Channel Flow: Problem Solving

248
To calculate the flow rate for a trapezoidal channel, first, identify the bottom width, side slope, and flow depth of the channel. The cross-sectional area (A) corresponding to the depth of flow (y), channel bottom width (B), and side slope (θ) is determined by:Next, calculate the wetted perimeter, which includes the bottom width and the sloped side lengths in contact with the water. Using the values of the cross-sectional area and the wetted perimeter, determine the hydraulic radius by...
248

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes of lateral and posterior approaches in hip arthroplasty: A cohort from low-middle-income country.

JPMA. The Journal of the Pakistan Medical Association·2026
Same author

30-day morbidity and mortality after hip surgery - A single-centre study.

JPMA. The Journal of the Pakistan Medical Association·2026
Same author

Cardiac Arrest as the First Manifestation of Single Coronary Artery-Potential Role of FFR<sub>CT</sub> for Detecting Critical Flow Reduction. A Case Report.

Clinical case reports·2026
Same author

VerbaFake, EchoFake, and PixelFake: lightweight architectures with advanced augmentation for unimodal deepfake detection.

Scientific reports·2026
Same author

Core-shell nanofiber encapsulation of α-linolenic acid via peanut protein isolate-pullulan Maillard conjugates: Enhanced oxidative stability and bioaccessibility.

Carbohydrate polymers·2026
Same author

Sexualized drug use, anxiety, depression, and suicidal ideation among men who have sex with men in Pakistan: Results of a multi-center cross-sectional survey.

PLOS global public health·2026
Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026
Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026
Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026
Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026
Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026
Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026
See all related articles

Related Experiment Video

Updated: Nov 20, 2025

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

9.8K

Depth enhancement through correlation-based guided filtering in shape from focus.

Usman Ali1, Muhammad Tariq Mahmood1,2

  • 1Computer Engineering, School of Computer Science and Engineering, Korea University of Technology and Education, Cheonan, Republic of Korea.

Microscopy Research and Technique
|January 25, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel depth enhancement method for shape-from-focus (SFF) techniques. By incorporating a guidance map and guided filtering, the method significantly improves depth map accuracy and detail reconstruction.

Keywords:
focus measure operatorguided filteringmicrostructuresregularizationshape from focus

More Related Videos

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.5K
Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

16.7K

Related Experiment Videos

Last Updated: Nov 20, 2025

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

9.8K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.5K
Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

16.7K

Area of Science:

  • Computer Vision
  • Image Processing
  • 3D Reconstruction

Background:

  • Traditional shape-from-focus (SFF) methods often lack accuracy due to insufficient use of image sequence information.
  • Reconstructed depth maps can suffer from inaccuracies and loss of fine structural details without proper guidance.

Discussion:

  • A novel guidance map is computed using correlations within image and focus volumes.
  • Guided filtering integrates this guidance map to refine an initial depth map derived from focus measures.
  • This approach enhances depth accuracy by leveraging inter-image relationships.

Key Insights:

  • The proposed method effectively enhances depth map quality by incorporating a novel guidance map.
  • Experimental results show significant improvements in reconstructed depth maps for various image sequences.
  • The technique addresses limitations of conventional SFF by utilizing image volume correlations.

Outlook:

  • Further research could explore adaptive guidance map generation for dynamic scenes.
  • Integration with other 3D reconstruction modalities may yield synergistic benefits.
  • Potential applications include enhanced microscopy, robotics, and augmented reality systems.