Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Radiologist-AI Collaboration for Ischemia Diagnosis in Small-Bowel Obstruction: Multicentric Development and External Validation of a Multimodal Deep Learning Model.

Journal of imaging informatics in medicine·2026

Same author

A proximal algorithm for joint blood flow computation and tissue motion compensation in Doppler ultrafast ultrasound imaging.

Ultrasonics·2026

Same author

Multiscale insights into biofilm development on hydrophobic fouling-release coatings.

Scientific reports·2026

Same author

Deep Probabilistic Matrix Factorization on Graphs: Application to Drug Repositioning in Antimicrobial Resistance.

IEEE transactions on computational biology and bioinformatics·2025

Same author

Genetic deregulation of REL in germinal center B cells induces generation of a pool of lymphoma precursor cells.

Blood advances·2025

Same author

Photodynamic Therapy against Colorectal Cancer Using Porphin-Loaded Arene Ruthenium Cages.

International journal of molecular sciences·2024

Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026

Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026

Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026

Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026

Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026

Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 20, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Multiplex-multiphoton microscopy and computational strategy for biomedical imaging.

Thomas Hortholary^1,2, Claire Carrion³, Emilie Chouzenoux⁴

¹CNRS UMR 7252, XLIM Research Institute, Université de Limoges, Limoges, France.

Microscopy Research and Technique

|January 25, 2021

Summary

This summary is machine-generated.

A novel supercontinuum laser strategy enables multiplex-multiphoton microscopy (M-MPM) for efficient, one-shot imaging of diverse biological samples. This advancement simplifies excitation, offering broad applicability in biomedical research without parameter adjustments.

Keywords:

PSF estimation biomedical imaging computational strategy multiphoton microscopy supercontinuum lasers

More Related Videos

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Related Experiment Videos

Last Updated: Nov 20, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Area of Science:

Biomedical Optics
Microscopy Technology
Laser Physics

Background:

Multiphoton microscopy (MPM) is crucial for deep tissue imaging.
Current MPM systems often require complex excitation parameter adjustments for different fluorophores.
A simplified, versatile excitation source is needed for broader applications.

Purpose of the Study:

To introduce a novel, simplified laser strategy for multiplex-multiphoton microscopy (M-MPM).
To demonstrate the capability of M-MPM for one-shot imaging of diverse biological samples.
To evaluate the imaging resolution of the M-MPM system.

Main Methods:

Utilized a turn-key supercontinuum laser with a spectral shaping module for excitation.
Developed a multiplex-multiphoton microscopy (M-MPM) device.
Employed the computational approach FIGARO for point-spread-function (PSF) assessment.

Main Results:

Successfully performed one-shot microscopic imaging of various biomedical and vegetal models.
Demonstrated M-MPM's polyvalence with constant spectral excitation parameters.
Quantified PSF width, showing values comparable to standard optical microscopy.

Conclusions:

The supercontinuum laser strategy offers a significant instrumental advancement for biomedical MPM.
M-MPM enables efficient imaging of multiple fluorophores with a single excitation setup.
The developed system simplifies complex imaging tasks, enhancing accessibility in biological research.