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Transmission Electron Microscopy

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...

Atomic Emission Spectroscopy: Instrumentation

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers. Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.

Interaction of EM Radiation with Matter: Spectroscopy

Interaction of EM Radiation with Matter: Spectroscopy

Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Overview of Electron Microscopy

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....

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Related Experiment Video

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Complementary LEEM and eV-TEM for imaging and spectroscopy.

Peter S Neu¹, Daniël Geelen¹, Aniket Thete¹

¹Huygens-Kamerlingh Onnes Laboratory, Leiden Institute of Physics, Leiden University, Niels Bohrweg 2, Leiden, The Netherlands.

Ultramicroscopy

|January 25, 2021

Summary

This summary is machine-generated.

This study introduces a modified low-energy electron microscopy (LEEM) technique for damage-free imaging of biological samples. The enhanced electron microscopy achieves nanometer resolution, preserving delicate structures even under prolonged electron illumination.

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Area of Science:

Materials Science
Biophysics
Electron Microscopy

Background:

Conventional transmission electron microscopy (TEM) can damage delicate biological samples due to high-energy electrons.
Low-energy electron microscopy (LEEM) offers a potential solution for minimizing sample damage.

Purpose of the Study:

To develop and demonstrate a modified LEEM technique for damage-free imaging of biological samples at nanometer resolution.
To enable imaging and spectroscopy in both transmission and reflection modes using low-energy electrons.

Main Methods:

Integration of a second electron source into a low-energy electron microscopy (LEEM) setup.
Operation in the 0-30 eV electron energy range for imaging and spectroscopy.
Experimental demonstration on free-standing graphene, gold nanoparticles on graphene, and DNA origami on graphene oxide.

Main Results:

Achieved nanometer resolution imaging in both transmission and reflection modes.
Demonstrated experimental imaging of graphene, gold nanoparticles, and DNA origami.
Showcased the resilience of DNA origami structures to prolonged low-energy electron illumination, preserving discernable features.

Conclusions:

The enhanced low-energy electron microscopy (eV-TEM) technique enables damage-free imaging of biological samples at nanometer resolution.
The technique is versatile, applicable to various samples including graphene and DNA origami, and compatible with advanced 2D membrane sample preparation.
eV-TEM represents a significant advancement for high-resolution imaging of sensitive biological materials.