Related Experiment Video
Updated: Jun 28, 2026

Single Molecule Analysis of Laser Localized Psoralen Adducts
Published on: April 20, 2017
Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts
R D Wood1, P Robins, T Lindahl
1Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, England.
Abstract:
Soluble extracts from human lymphoid cell lines that perform repair synthesis on covalently closed circular DNA containing pyrimidine dimers or psoralen adducts are described. Short patches of nucleotides are introduced by excision repair of damaged DNA in an ATP-dependent reaction. Extracts from xeroderma pigmentosum cell lines fail to act on damaged circular DNA, but are proficient in repair synthesis of ultraviolet-irradiated DNA containing incisions generated by Micrococcus luteus pyrimidine dimer-DNA glycosylase. Repair is defective in extracts from all xeroderma pigmentosum cell lines investigated, representing the genetic complementation groups A, B, C, D, H, and V. Mixing of cell extracts of group A and C origin leads to reconstitution of the DNA repair activity.
More Related Videos
08:18Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Published on: September 5, 2017
11:58A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Published on: March 5, 2018
Related Concept Videos
Nucleotide Excision Repair
Base Excision Repair
The first step of...
Long-patch Base Excision Repair
Nucleotide Excision Repair
Base Excision Repair
The first step of...
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...