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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Nov 20, 2025

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

Rhodamines with a Chloronicotinic Acid Fragment for Live Cell Superresolution STED Microscopy*.

Florian Grimm¹, Jasmin Rehman¹, Stefan Stoldt²

¹Abberior GmbH, Hans Adolf Krebs Weg 1, 37077, Göttingen, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|January 26, 2021

Summary

This summary is machine-generated.

New rhodamine dyes were synthesized from nicotinic acid derivatives, offering specific labeling of cellular structures. These fluorescent dyes enable advanced two-color STED microscopy for detailed biological imaging.

Keywords:

dyes/pigments fluorescence fluorescent probes scanning probe microscopy

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Last Updated: Nov 20, 2025

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

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Ground State Depletion Super-resolution Imaging in Mammalian Cells

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Published on: November 5, 2017

Area of Science:

Organic Chemistry
Biophysical Chemistry
Cell Biology

Background:

Fluorescent dyes are essential tools for visualizing biological structures and processes.
Existing dyes like TAMRA have limitations in specificity and spectral properties.
Development of novel fluorescent probes is crucial for advanced microscopy techniques.

Purpose of the Study:

To synthesize novel rhodamine-based fluorescent dyes with tunable spectral properties.
To evaluate the dyes' performance in labeling cellular organelles and cytoskeleton components.
To demonstrate the application of these dyes in two-color STED microscopy.

Main Methods:

Formylation of 2,6-dichloro-5-R-nicotinic acids followed by condensation with 3-hydroxy-N,N-dimethylaniline.
Selective reaction with thioglycolic acid to yield new rhodamine derivatives.
Conjugation of dyes with small molecules for cellular labeling and application in STED microscopy.

Main Results:

Successful synthesis of novel rhodamine dyes with absorption/emission maxima at 564/584 nm (R=H) and 573/597 nm (R=F).
Specific covalent and non-covalent labeling of organelles and cytoskeleton in living cells.
Effective combination with other fluorescent probes for two-color STED microscopy.

Conclusions:

The synthesized rhodamine dyes offer distinct spectral properties and high specificity for cellular labeling.
These novel probes are suitable for advanced imaging applications, including multi-color STED microscopy.
The study expands the toolkit for live-cell imaging and super-resolution microscopy.