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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Updated: Nov 20, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Aptamer-Linked CRISPR/Cas12a-Based Immunoassay.

Hui Li¹, Menglu Li², Yucai Yang³

¹School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P. R. China.

Analytical Chemistry

|January 26, 2021

Summary

This summary is machine-generated.

This study introduces a novel method using CRISPR/Cas12a and aptamer-linked DNA activators for ultrasensitive detection of non-nucleic acid biomarkers. This approach enhances versatility and simplifies operation for broader clinical applications in diagnostics.

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Area of Science:

Biotechnology
Molecular Biology
Biosensing

Background:

The CRISPR/Cas system shows potential in nucleic acid biosensing.
Current limitations include low versatility, moderate sensitivity, and complex operations for non-nucleic acid targets, hindering clinical translation.

Purpose of the Study:

To develop a versatile and sensitive detection method for non-nucleic acid analytes using the CRISPR/Cas12a system.
To overcome the limitations of existing CRISPR-based biosensing platforms for clinical diagnostics.

Main Methods:

Designed aptamer-flanked activator DNA strands to link non-nucleic acid analytes to the CRISPR/Cas12a system.
Utilized the collateral cleavage activity of CRISPR/Cas12a for signal amplification.
Adapted the sensing principle to be compatible with existing enzyme-linked immunosorbent assay (ELISA) platforms.

Main Results:

Achieved ultrasensitive detection of biomarkers from various species.
Demonstrated a direct correlation between non-nucleic acid analytes and CRISPR/Cas12a activation.
The system offers optional signal output and compatibility with ELISA platforms.

Conclusions:

The developed method significantly broadens the applicability of CRISPR/Cas systems in bioanalysis and medical diagnostics.
This approach facilitates the clinical transition of CRISPR-based biosensing technologies by enhancing sensitivity, versatility, and ease of use.