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Sox9 function in salivary gland development.

Junichi Tanaka1, Koki Takamatsu1, Akane Yukimori1

  • 1Division of Pathology, Department of Oral Diagnostic Sciences, Showa University School of Dentistry, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan.

Journal of Oral Biosciences
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Sox9 is a key transcription factor essential for salivary gland development in mice. Understanding the Sox9 pathway offers potential for regenerating damaged salivary glands.

Keywords:
ChIP-seqOrganoidsSalivary glandsStem cellsTranscription factors

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Organogenesis relies on specific transcription networks, necessitating identification of organ-specific pathways for understanding development and regeneration.
  • Mouse submandibular glands (SMGs) develop from oral epithelium invagination, with the Fgf10 and Sox9 network playing a role.

Purpose of the Study:

  • To investigate the role of Sox9 in salivary gland development and its potential for regeneration.
  • To identify the molecular mechanisms underlying salivary gland organogenesis.

Main Methods:

  • Analysis of Sox9 expression during mouse SMG development.
  • Generation and analysis of conditional knockout (cKO) mice lacking Sox9 in oral epithelium.
  • Organoid culture system using adenoviral transduction of Sox9 and Foxc1.
  • ChIP-sequencing to identify Sox9-regulated genes.

Main Results:

  • Sox9 is expressed throughout mouse SMG development, with altered distribution.
  • Conditional deletion of Sox9 in oral epithelium resulted in smaller SMG buds.
  • Adenoviral transduction of Sox9 and Foxc1 induced salivary gland rudiments in vitro.
  • ChIP-sequencing suggested Sox9 regulates genes involved in tube and branching formation.

Conclusions:

  • Sox9 is a critical transcription factor for salivary gland development.
  • The Sox9-mediated pathway presents a promising target for salivary gland regeneration strategies.