Lentzea alba sp. nov., a novel actinobacterium isolated from soil

A novel actinobacterium, designated strain NEAU-D13^T, was isolated from soil collected from Aohan Banner, Chifeng, Inner Mongolia Autonomous Region, China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-D13^T belonged to the genus Lentzea and shared the highest sequence similarity with Lentzea kentuckyensisJCM 14913^T (99.17 %). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Lentzea. Cell walls contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were ribose and mannose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and phosphatidylinositol. The menaquinone was only MK-9(H₄). The major fatty acids were iso-C_16:0, C_16:0, anteiso-C_17:0, iso-C_15:0 and anteiso-C_15:0. DNA G+C content was 68.71 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that the strain formed a stable clade with L. kentuckyensisJCM 14913^T in the genus Lentzea. Meanwhile, a combination of digital DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-D13^T could be distinguished from its closely related strain. Therefore, it is concluded that strain NEAU-D13^T represents a novel species of the genus Lentzea, for which the name Lentzea alba sp. nov. is proposed, with NEAU-D13^T (=CCTCC AA 2019089^T=JCM 33970^T) as the type strain.