Related Experiment Videos
Forensic application of blood stains and polymorphism of the sixth component of human complement (C6)
K Kishi1, K Sawazaki, T Yasuda
1Department of Legal Medicine, Fukui Medical School, Japan.
Insights
This study determined complement component 6 (C6) allele frequencies in Japan. C6 phenotyping from aged blood stains is reliable, even with minimal sample volume.
Area of Science:
- Forensic Science
- Genetics
- Immunology
Background:
- Complement component 6 (C6) is crucial for the membrane attack complex formation.
- Accurate C6 allele frequency data is essential for population genetics and forensic applications.
- Previous studies have not comprehensively analyzed C6 allele frequencies in the Japanese population.
Purpose of the Study:
- To determine the gene frequencies of common C6 alleles in a Japanese population from Fukui prefecture.
- To assess the feasibility of C6 phenotyping from aged blood stains for forensic investigations.
Main Methods:
- Blood samples from 340 unrelated individuals were analyzed.
- Gene frequencies for C6 alleles (A, B, B2) were calculated.
- C6 phenotyping was performed on blood stains aged for over one year.
Main Results:
- The calculated gene frequencies were C6 A (0.478), C6 B (0.464), C6 B2 (0.052), and rare alleles (0.006).
- Successful C6 phenotyping was achieved from blood stains aged for one year.
- Less than 2 microliters of detectable whole blood was sufficient for phenotyping after aging.
Conclusions:
- Established C6 allele frequencies for the studied Japanese population.
- Validated the utility of C6 phenotyping from aged blood stains in forensic science.
- Demonstrated the robustness of C6 analysis even with degraded samples.
Abstract:
Blood samples from 340 unrelated individuals in Fukui prefecture in the central part of Japan were tested in order to determine the gene frequencies of the C6 common alleles. The gene frequencies calculated were as follows: C6 A, 0.478, C6 B, 0.464, C6 B2, 0.052 and rare alleles, 0.006. It was demonstrated that C6 phenotyping from blood stains aged over a period of 1 year, could be performed correctly. The quantity of detectable whole blood after this period amounted to less than 2 microliter.