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Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
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Andrographolide: Regulating the Master Regulator NF-κB.

K P Mishra1

  • 1Present Address: Defence Research and Development Organization (DRDO)- HQ, New Delhi, 110011 India.

Indian Journal of Clinical Biochemistry : IJCB
|January 28, 2021
PubMed
Summary
This summary is machine-generated.

Natural compounds like andrographolide show promise for inflammatory disorders by inhibiting the transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells). This natural compound acts as a master regulator inhibitor, offering new therapeutic avenues.

Keywords:
AndrographolideChemokinesCytokinesInflammationNatural products

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Area of Science:

  • Pharmacology
  • Natural Products Chemistry
  • Molecular Biology

Background:

  • Traditional Indian medicine has long utilized natural products, with established efficacy but often lacking modern mechanistic explanations.
  • Plant-derived compounds like artemisinin and taxol are crucial in modern medicine, particularly for inflammatory disorders and cancer.
  • The transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a key regulator of inflammatory gene expression.

Purpose of the Study:

  • To investigate the mechanism of action of plant-derived natural compounds in inflammatory processes.
  • To explore the potential of andrographolide as a therapeutic agent for inflammatory disorders.
  • To elucidate the role of andrographolide in regulating the NF-κB signaling pathway.

Main Methods:

  • Literature review on traditional medicine and modern pharmacology of natural products.
  • Analysis of the molecular mechanism involving transcription factor NF-κB.
  • Identification of plant-derived compounds with anti-inflammatory properties.

Main Results:

  • Andrographolide, a plant-derived compound, was identified as a potent inhibitor of inflammatory processes.
  • Andrographolide directly inhibits the binding of NF-κB to the DNA promoter sites of inflammatory genes.
  • This inhibition effectively suppresses the secretion of proinflammatory cytokines and chemokines.

Conclusions:

  • Andrographolide's ability to inhibit NF-κB positions it as a significant regulator of master regulatory pathways in inflammation.
  • Natural products, exemplified by andrographolide, offer promising therapeutic strategies for managing inflammatory diseases.
  • Further research into natural compounds can bridge traditional knowledge with modern scientific understanding for novel drug development.