Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon

Area of Science:

• Exercise Physiology
• Biomarker Discovery
• Cellular Biology

Background:

• Cellular nanovesicles (CNVs) are shed from cells and show promise as health indicators.
• The impact of intense physical activity on CNV concentration is not well understood.

Purpose of the Study:

• To investigate the effect of long-distance running on CNV concentration.
• To analyze changes in standard blood parameters following physical exertion.
• To explore the potential of CNVs and other markers as indicators of physiological response to exercise.

Main Methods:

• CNVs isolated via centrifugation and analyzed by flow cytometry.
• Cholinesterase (ChE) and glutathione S-transferase (GST) activity measured spectrophotometrically.
• Cytokine (IL-6, TNF-α), CRP, and lipidogram parameters assessed using ELISA and enzymatic assays.

Main Results:

• A significant decrease in CNV concentration (>15 hours post-exercise) was observed (46%, p<0.05).
• ChE activity, GST activity in erythrocytes, and IL-6 concentration also decreased significantly post-exercise.
• Strong correlations found between CNV concentration and ChE/GST activity in erythrocyte suspension.

Conclusions:

• Post-exercise, CNV concentration, ChE/GST activity, and IL-6 levels were reduced, indicating a favorable health effect of physical exertion.
• CNV concentration and ChE activity show potential as biomarkers for inflammation response after physical effort.
• Physical activity status is crucial for interpreting CNV-based diagnostic and therapeutic procedures.