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Updated: Aug 6, 2026
05:30
Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
Published on: January 31, 2025
High-Throughput Equine Plasma Profiling Using Laser-Assisted Rapid Evaporative Ionisation Mass Spectrometry
Adrienn Molnár1,2, Matevž Arko3, Kaja Troha4
1MTA-ELTE Lendület (Momentum) Ion Mobility Mass Spectrometry Research Group, Faculty of Science, Institute of Chemistry, ELTE Eötvös Loránd University, Budapest, Hungary.
Journal of Mass Spectrometry : JMS
|July 23, 2026
Summary
Therapeutic plasma quality can be rapidly assessed using laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS). This method links lipid profiles to platelet content, aiding in plasma stability and quality control for regenerative medicine applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Regenerative Medicine
Background:
- Therapeutic plasma, rich in platelets and extracellular vesicles (EVs), is vital for regenerative medicine, particularly in wound healing.
- Optimal plasma preparation and quality control are crucial but currently lack rapid, accurate analytical methods.
- Existing methods for plasma analysis are often time-consuming and require extensive sample preparation.
Purpose of the Study:
- To develop and validate a novel, rapid, and automated method for equine plasma profiling.
- To investigate the influence of sample preparation on plasma composition using lipidomic and metabolic analyses.
- To establish correlations between plasma molecular composition, platelet content, and EV levels.
Main Methods:
- Utilized a new automated well-plate sampling system with laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS) for plasma analysis.
- Performed comprehensive lipidomic and metabolic profiling within seconds, requiring minimal sample preparation.
- Quantified platelet and cellular fragment (P2 particle) number density via flow cytometry (FCM) and EVs via interferometric light microscopy (ILM).
- Correlated lipid profiles with platelet and EV content, and visualized samples using scanning electron microscopy (SEM).
Main Results:
- LA-REIMS provided rapid, comprehensive lipidomic and metabolic insights into plasma composition.
- Specific phosphatidylcholines and fatty acids were identified as sensitive indicators of plasma stability, affected by centrifugation parameters.
- A significant correlation was found between platelet (P2 particle) number density and plasma lipid profiles, but not with EV content.
- The study demonstrated the potential of LA-REIMS for high-throughput plasma analysis and quality control.
Conclusions:
- The developed LA-REIMS platform offers a fast and effective solution for analyzing plasma composition and quality.
- Platelets play a critical role in determining the molecular composition of therapeutic plasma.
- This technology has significant potential for quality control, diagnostics, and validating plasma prior to therapeutic use in regenerative medicine.
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