Circ_0105346 Knockdown Inhibits Osteosarcoma Development via Regulating miR-1182/WNT7B Axis

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Osteosarcoma (OS) is a prevalent bone cancer in pediatric patients.
• Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
• This study investigates the function of circ_0105346 in OS.

Purpose of the Study:

• To elucidate the role of circRNA circ_0105346 in osteosarcoma (OS) development.
• To explore the underlying molecular mechanism involving microRNA (miR)-1182 and wingless-type MMTV integration site family 7B (WNT7B).

Main Methods:

• Quantitative reverse transcription PCR (qRT-PCR) and Western blot to assess expression levels.
• Cell proliferation, apoptosis, cell cycle, migration, and invasion assays (MTT, flow cytometry, transwell, wound healing).
• Dual-luciferase reporter, RNA immunoprecipitation (RIP), and pull-down assays to confirm molecular interactions; in vivo mouse xenograft model.

Main Results:

• Circ_0105346 and WNT7B were upregulated, while miR-1182 was downregulated in OS tissues and cells.
• Circ_0105346 knockdown inhibited OS cell proliferation, migration, invasion, and glycolysis, and promoted apoptosis.
• Circ_0105346 targets miR-1182, which targets WNT7B, establishing a circ_0105346/miR-1182/WNT7B regulatory axis; tumor growth was inhibited in vivo.

Conclusions:

• Circ_0105346 knockdown suppresses osteosarcoma progression.
• The circ_0105346/miR-1182/WNT7B axis is a key regulator of OS development.
• Circ_0105346 represents a potential therapeutic target for osteosarcoma.