Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a spatially defined 3D<i>in vitro</i>coculture construct modeling pancreatic cancer-associated cachexia.

Biofabrication·2026
Same author

Correction: Pan et al. Therapeutic Potential of BMX-001 for Preventing Chemotherapy-Induced Peripheral Neuropathic Pain. <i>Pharmaceuticals</i> 2025, <i>18</i>, 1159.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Engineering Resatorvid-Loaded Sub-Microgels of Epigallocatechin-3-gallate/Hyaluronic Acid to Treat Acute Lung Injury.

Advanced healthcare materials·2026
Same author

Contribution of astrocytic calcium signaling to auditory hypersensitivity in a mouse model of fragile X syndrome.

Neurobiology of disease·2026
Same author

The Effects of Ovine-Derived Reinforced Tissue Matrix Surrounding Silicone-Based Implants in a Rat Prepectoral Reconstruction Model.

Bioengineering (Basel, Switzerland)·2026
Same author

Enhanced Primary Motor Cortex Astrocyte Calcium Signaling With Motor Learning.

Neural plasticity·2026

Related Experiment Video

Updated: Nov 19, 2025

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
10:17

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering

Published on: May 16, 2022

2.5K

3D Printed Hydrogels with Aligned Microchannels to Guide Neural Stem Cell Migration.

Cui Li1,2, Mitchell Kuss3, Yunfan Kong3

  • 1Department of Physiology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China.

ACS Biomaterials Science & Engineering
|January 28, 2021
PubMed
Summary

Researchers developed 3D printed hydrogel scaffolds to guide neural stem/progenitor cells (NSCs) for brain tissue repair after injury. These scaffolds promote organized cell integration, crucial for functional recovery in the cerebral cortex.

Keywords:
aligned microchannelbrain injurydigital light processinggrowth factor gradient

More Related Videos

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
07:48

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Published on: May 5, 2023

1.6K
Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
08:52

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

14.7K

Related Experiment Videos

Last Updated: Nov 19, 2025

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
10:17

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering

Published on: May 16, 2022

2.5K
Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs
07:48

Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

Published on: May 5, 2023

1.6K
Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
08:52

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

14.7K

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Regenerative Medicine

Background:

  • Brain injuries cause cell death and cavity formation, leading to neurological deficits.
  • Neural stem/progenitor cell (NSC) transplantation is a promising therapy for brain repair.
  • Organized integration of transplanted cells, especially in the cerebral cortex, is crucial for successful therapy.

Purpose of the Study:

  • To develop a biomaterial-based strategy for guiding transplanted NSCs in brain tissue regeneration.
  • To create 3D printed hydrogel scaffolds with precise shape, aligned microchannels, and tunable mechanical properties.
  • To enhance the organization and integration of NSCs at the lesion site.

Main Methods:

  • Utilized a digital light-processing-based 3D printer to fabricate hydrogel scaffolds.
  • Designed scaffolds with uniaxially aligned microchannels and tunable, brain tissue-matching mechanical properties.
  • Incorporated spatial control of bioactive molecule distribution within the scaffolds.

Main Results:

  • Achieved high shape precision for lesion site reconstruction.
  • Demonstrated excellent neuro-compatibility with aligned neuronal outgrowth along microchannels.
  • Validated the capacity of scaffolds to guide NSC organization and integration.

Conclusions:

  • 3D printed hydrogel scaffolds offer a promising approach for brain tissue regeneration.
  • These scaffolds can act as a protective and guidance vehicle for transplanted NSCs.
  • The developed biomaterial strategy facilitates organized cell engraftment and integration for improved functional recovery.