Effect of nicotine on transferrin binding and iron uptake by cultured rat placenta

The effect of nicotine on transferrin and iron transport in placental cells has been studied. Nicotine inhibits iron uptake but has little effect on the steady-state levels of transferrin. The effect is temperature and concentration dependent and is not reversible. At a concentration of 15 mM nicotine inhibited transferrin endocytosis by 40%, while iron uptake was decreased by nearly 60%. Nicotine exerted a similar effect on reticulocytes, but other amines, either tertiary or quaternary, had little or no effect on either iron uptake or steady-state intracellular transferrin levels. The results suggest that nicotine acts by blocking uptake, probably by acting as a weak base inhibiting iron release from transferrin, and inhibiting exocytosis with a resultant block of endocytosis. The concentrations required to exert an effect are too high to implicate inhibition of iron transport in the effects of smoking on pregnancy.