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Manool, a diterpene from Salvia officinalis, exerts preventive effects on chromosomal damage and preneoplastic

Heloiza Diniz Nicolella1, Gabriela Fernandes1, Saulo Duarte Ozelin1

  • 1Mutagenesis Laboratory, Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil.

Mutagenesis
|January 29, 2021
PubMed

Insights

Manool, a diterpene, demonstrates antigenotoxic and anticarcinogenic properties by reducing DNA damage and colon tumors. Its anti-inflammatory effects, particularly on nitric oxide production, may contribute to its chemopreventive potential.

Area of Science:

  • Pharmacology and Toxicology
  • Cancer Research
  • Molecular Biology

Background:

  • Genomic integrity is crucial for preventing diseases like cancer.
  • Diterpenes are natural compounds with potential therapeutic applications.
  • Understanding the chemopreventive mechanisms of natural products is vital.

Purpose of the Study:

  • To evaluate the antigenotoxic and anticarcinogenic effects of manool.
  • To investigate the influence of manool on genotoxicity induced by various mutagens.
  • To explore the anti-inflammatory mechanisms underlying manool's chemopreventive activity.

Main Methods:

  • In vitro genotoxicity assays using V79 cells exposed to mutagens (doxorubicin, hydrogen peroxide, etoposide).
  • In vivo studies in mice to assess chromosomal damage and in rats for colon carcinogenesis.
  • Analysis of serum biochemical indicators for hepatotoxicity and nephrotoxicity.
  • Evaluation of nitric oxide (NO) production and NF-kB gene expression in response to manool treatment.

Main Results:

  • Manool significantly reduced micronuclei formation induced by doxorubicin and hydrogen peroxide in V79 cells.
  • A significant reduction in doxorubicin-induced chromosomal damage was observed in mice treated with manool.
  • Manool exhibited anticarcinogenic effects against chemically induced preneoplastic lesions in rat colon.
  • Manool modulated nitric oxide production and did not show hepatotoxicity or nephrotoxicity.

Conclusions:

  • Manool possesses significant antigenotoxic and anticarcinogenic properties.
  • The anti-inflammatory potential of manool, particularly its effect on NO, may contribute to its chemopreventive activity.
  • Further research into manool's mechanisms could lead to novel cancer prevention strategies.

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