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Updated: Nov 19, 2025
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
Published on: November 28, 2018
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Transcriptome analysis reveals the long intergenic noncoding RNAs contributed to skeletal muscle differences between
Ziying Huang1, Qianqian Li1, Mengxun Li1
1Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China.
Scientific Reports
|January 30, 2021
Summary
Long intergenic noncoding RNAs (lincRNAs) may explain differences in skeletal muscle growth between pig breeds. This study identified key lincRNAs and their target genes involved in muscle development pathways.
Area of Science:
- Genomics
- Molecular Biology
- Animal Science
Background:
- Skeletal muscle growth rates vary significantly between Western and domestic pig breeds.
- Long intergenic noncoding RNAs (lincRNAs) are recognized as crucial regulators of skeletal muscle development.
Purpose of the Study:
- To investigate the role of lincRNAs in the divergent skeletal muscle development between Yorkshire (LW) and Tibetan pigs (TP).
- To identify differentially expressed lincRNAs (DELs) and their potential target genes (PTGs) associated with muscle growth differences.
Main Methods:
- Comparative transcriptome analysis of LW and TP skeletal muscle data.
- Prediction of DELs, PTGs, and quantitative trait loci (QTLs).
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.
- Construction of a DEL-PTG co-expression network.
Main Results:
- Identified 138 DELs between LW and TP breeds.
- PTGs were enriched in muscle development-related biological processes and pathways.
- Most DELs' QTLs were associated with muscle development traits.
- Significant correlations were found between DELs and muscle development-related PTGs (MDRPTGs).
- MDRPTGs are implicated in the IGF-1-AKT-mTOR pathway, crucial for muscle fiber hypertrophy.
Conclusions:
- This study identified potential lincRNAs contributing to skeletal muscle development differences in pigs.
- The findings provide insights into the regulatory mechanisms of lincRNA in muscle growth.
- These results offer a valuable reference for future research on lincRNA's role in skeletal muscle development.
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