Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine learning-augmented lateral flow assays for point-of-care infectious disease diagnostics.

Lab on a chip·2026
Same author

Peptide-Based Fluorescent Biosensing System for the Detection of the Melanoma Biomarker S100B.

Bioconjugate chemistry·2025
Same author

Optical Contact Lenses Biosensors.

ACS sensors·2025
Same author

Diagnostic technologies for neuroblastoma.

Lab on a chip·2025
Same author

Synthesis and study of amorphous calcium phosphate dual-targeted drug-carrying platforms.

Journal of materials chemistry. B·2025
Same author

Engineering in vitro vascular microsystems.

Microsystems & nanoengineering·2025

Related Experiment Video

Updated: Nov 19, 2025

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
11:01

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

Published on: January 11, 2012

16.8K

Pentapeptide IKVAV-engineered hydrogels for neural stem cell attachment.

Yixia Yin1, Wenwu Wang1, Qi Shao1

  • 1State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China. tongttqq@whut.edu.cn.

Biomaterials Science
|January 30, 2021
PubMed
Summary

This study developed a novel IKVAV-functionalized hydrogel to support neural stem cell growth for spinal cord injury repair. The biodegradable scaffold promotes cell survival, proliferation, and differentiation, offering a promising approach for neural tissue engineering.

More Related Videos

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

9.5K
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.6K

Related Experiment Videos

Last Updated: Nov 19, 2025

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
11:01

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

Published on: January 11, 2012

16.8K
Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
11:37

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Published on: February 3, 2018

9.5K
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.6K

Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Regenerative Medicine

Background:

  • Spinal cord injury (SCI) is currently irreversible due to the lack of neuronal regeneration.
  • Neural tissue engineering using scaffolds and neural stem cells offers a potential therapeutic strategy for SCI repair.
  • Neural cells require microenvironments similar to the extracellular matrix for optimal support and survival.

Purpose of the Study:

  • To develop a novel three-dimensional (3D) hydrogel scaffold functionalized with the IKVAV pentapeptide.
  • To evaluate the biocompatibility and neural stem cell (NSC) support capabilities of the IKVAV-functionalized hydrogel.
  • To assess the in vivo performance of the hydrogel for potential SCI regenerative applications.

Main Methods:

  • Fabrication of a 3D pentapeptide IKVAV-functionalized poly(lactide ethylene oxide fumarate) (PLEOF) hydrogel.
  • In vitro assessment of hydrogel biodegradability, hemo-biocompatibility, and support for NSC attachment, growth, proliferation, and differentiation.
  • In vivo implantation of hydrogels to evaluate biodegradability, biocompatibility, vascularization, and inflammatory response.

Main Results:

  • The IKVAV-PLEOF hydrogels demonstrated biodegradability and hemo-biocompatibility in vitro.
  • The hydrogels effectively supported NSC attachment, growth, proliferation, and differentiation.
  • NSCs readily formed spheroids that were successfully encapsulated and proliferated within the 3D hydrogel constructs.
  • In vivo tests confirmed hydrogel biodegradability, biocompatibility, vascularization, and minimal inflammatory response after 4 weeks.

Conclusions:

  • The IKVAV-PLEOF hydrogel serves as a promising biomaterial for neural tissue engineering applications.
  • This scaffold supports neural stem cell spheroid encapsulation and promotes their survival and growth.
  • The developed hydrogel holds potential for advancing spinal cord injury repair and brain regeneration strategies.