Related Experiment Video

Updated: Nov 19, 2025

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.4K

SP1 induced long non-coding RNA AGAP2-AS1 promotes cholangiocarcinoma proliferation via silencing of CDKN1A

Hao Ji1,2, Juan Wang1,2, Binbin Lu1,2

  • 1Department of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, 210000, Jiangsu, People's Republic of China.

Molecular Medicine (Cambridge, Mass.)
|February 1, 2021
PubMed
Abstract

Insights

The long non-coding RNA AGAP2-AS1 promotes cholangiocarcinoma (CCA) proliferation. Its upregulation in CCA tissues suggests AGAP2-AS1 is a potential therapeutic target for this cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Long non-coding RNAs (LncRNAs) regulate gene expression through various mechanisms.
  • The specific roles of LncRNAs in cholangiocarcinoma (CCA) pathogenesis remain largely unknown.
  • Understanding LncRNA function in CCA is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the expression and function of the LncRNA AGAP2-AS1 in cholangiocarcinoma.
  • To elucidate the molecular mechanisms underlying AGAP2-AS1's role in CCA progression.

Main Methods:

  • Analysis of AGAP2-AS1 expression in 32 CCA tissues and two CCA cell lines.
  • In vitro experiments involving AGAP2-AS1 knockdown and overexpression.
  • Chromatin immunoprecipitation (CHIP) and RNA immunoprecipitation (RIP) assays to identify AGAP2-AS1 targets.

Main Results:

  • AGAP2-AS1 was significantly upregulated in CCA tumor tissues compared to normal tissues.
  • SP1 was identified as an inducer of AGAP2-AS1 expression in CCA.
  • Knockdown of AGAP2-AS1 inhibited CCA cell proliferation and induced apoptosis.
  • Overexpression of AGAP2-AS1 promoted CCA cell proliferation.

Conclusions:

  • The LncRNA AGAP2-AS1 is upregulated in cholangiocarcinoma and promotes tumor cell proliferation.
  • AGAP2-AS1 plays a significant role in CCA tumorigenesis.
  • Targeting AGAP2-AS1 may represent a novel therapeutic strategy for cholangiocarcinoma.
Keywords:
AGAP2-AS1Biomarker apoptosisCCAEZH2LncRNA

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.8K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.4K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.9K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.3K