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The Association of IL-6, TNFα and CRP Gene Polymorphisms with Coronary Artery Disease in a Tunisian Population: A

Nedra Grira1,2, Dhaker Lahidheb3,4, Oussama Lamine3,5

  • 1Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia. nedra.grira@fst.utm.tn.

Biochemical Genetics
|February 2, 2021
PubMed

Insights

Genetic variations in Interleukin-6 (IL-6), Tumor Necrosis Factor alpha (TNFα), and C-reactive protein (CRP) genes are associated with increased coronary artery disease (CAD) risk in the Tunisian population. These polymorphisms may serve as crucial susceptibility markers for CAD.

Area of Science:

  • Cardiovascular Genetics
  • Immunology
  • Population Health

Background:

  • Coronary artery disease (CAD) is recognized as an inflammatory condition.
  • Systemic inflammation markers like Interleukin-6 (IL-6), Tumor Necrosis Factor alpha (TNFα), and C-reactive protein (CRP) are linked to cardiovascular event risk.
  • Investigating genetic susceptibility markers for CAD is crucial for understanding disease etiology.

Purpose of the Study:

  • To evaluate the association of specific gene variants (IL-6 -174 G/C, TNFα -308 A/G, CRP +1059G/C) with CAD susceptibility in a Tunisian cohort.
  • To determine if these polymorphisms act as risk factors for developing coronary artery disease.

Main Methods:

  • A case-control study was conducted with 204 CAD patients and 400 controls from Tunisia.
  • Genotyping of IL-6, TNFα, and CRP gene variants was performed using polymerase chain reaction and restriction fragment length polymorphism analysis.
  • Clinical data including BMI, smoking history, lipid profiles, and inflammatory marker levels were analyzed.

Main Results:

  • Significant differences in BMI, family history of CAD, smoking, TC, LDLc, hs-CRP, IL6, and TNFα levels were observed between CAD patients and controls.
  • Specific genotypes showed significant differences: IL6-174CC (OR=7.71), TNFα -308 AA (OR=2.95), and CRP +1059 CC (OR=5.40) were associated with increased CAD risk.
  • No association was found between these genotypes and the measured levels of inflammatory markers.

Conclusions:

  • The IL-6 (-174 G/C), TNFα (-308 A/G), and CRP (+1059G/C) polymorphisms are identified as potential risk factors for coronary artery disease in the Tunisian population.
  • These genetic variations may contribute to CAD susceptibility independent of their direct impact on circulating inflammatory marker levels.

Keywords:
CRPCoronary artery diseaseGene polymorphismsIL 6Inflammatory markersTnfα

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