Related Experiment Video

Updated: Sep 2, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

31.9K

A PCR plus restriction enzyme-based technique for detecting target-enzyme mutations at position Pro-106 in

Hossein Ghanizadeh1, Andrew G Griffiths2, Christopher E Buddenhagen3

  • 1School of Agriculture and Environment, Massey University, Palmerston North, New Zealand.

Plos One
|February 2, 2021
PubMed

More Related Videos

Protocols for Robust Herbicide Resistance Testing in Different Weed Species10:52

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

Published on: July 2, 2015

14.9K
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

7.6K

Related Concept Videos

PCR02:07

PCR

The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce millions or billions of DNA copies within just a few hours. In a PCR reaction, a heat-resistant DNA polymerase enzyme amplifies the original DNA through a series of temperature changes inside an automated machine called a thermocycler.PCR is a Versatile Method that Revolutionized Molecular BiologyKary Mullis developed PCR in 1983, for which he was...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
PCR - Polymerase Chain Reaction02:07

PCR - Polymerase Chain Reaction

The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce millions or billions of DNA copies within just a few hours. In a PCR reaction, a heat-resistant DNA polymerase enzyme amplifies the original DNA through a series of temperature changes inside an automated machine called a thermocycler.PCR is a Versatile Method that Revolutionized Molecular BiologyKary Mullis developed PCR in 1983, for which he was...
Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...