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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

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Published on: November 7, 2012

Elevated temperature increases genome-wide selection on de novo mutations.

David Berger¹, Josefine Stångberg¹, Julian Baur¹

¹Department of Ecology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, 75236 Uppsala, Sweden.

Proceedings. Biological Sciences

|February 2, 2021

Summary

Rising global temperatures intensify natural selection by increasing DNA variation effects on protein stability. This impacts genetic diversity and evolution rates under climate change, affecting mutation load and fitness.

Keywords:

climate change enzyme kinetics mutation protein stability selection temperature

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Area of Science:

Evolutionary biology
Genetics
Climate change science

Background:

Adaptation relies on genetic variation and natural selection.
The impact of specific ecological factors on genetic variation and selection is unclear.

Purpose of the Study:

To investigate how rising global temperatures affect natural selection intensity.
To determine if environmental stress increases adaptation costs.

Main Methods:

Applied enzyme kinetic theory to predict temperature effects on DNA variation.
Estimated temperature-dependent fitness of mutations in seed beetles.
Calculated selection estimates on mutations across diverse organisms in varying environments.

Main Results:

Elevated temperatures intensify natural selection genome-wide by affecting protein stability.
Environmental stress alone did not increase selection on new mutations.
Increased temperature elevated selection on existing genetic polymorphism, raising mutation load and fitness variance.

Conclusions:

Rising temperatures enhance natural selection's strength, influencing evolution.
The cost of adaptation does not necessarily increase in novel environments.
Findings have implications for genetic diversity patterns and evolutionary predictability under climate change.