Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types of RNA01:23

Types of RNA

70.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
70.9K
Types of RNA01:20

Types of RNA

8.2K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
8.2K
RNA Interference01:23

RNA Interference

27.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.1K
Translational Regulation01:29

Translational Regulation

354
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
354
Riboswitches01:56

Riboswitches

9.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.1K
Experimental RNAi02:15

Experimental RNAi

6.8K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A unified photosensitizer platform for <i>in situ</i> DNA-, RNA-, and protein-directed proximity labeling.

bioRxiv : the preprint server for biology·2026
Same author

Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics.

Nature chemistry·2026
Same author

Lighting up lipid droplets.

Nature chemical biology·2026
Same author

A Scalable Design for Proximity-Inducing Molecules.

bioRxiv : the preprint server for biology·2026
Same author

Photosensitizer proximity labeling captures the lipid and protein interactomes.

Nature chemical biology·2026
Same author

Covalent chemical probes.

Communications chemistry·2025

Related Experiment Video

Updated: Nov 18, 2025

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.6K

New approaches to target RNA binding proteins.

Ashley R Julio1, Keriann M Backus2

  • 1Department of Chemistry and Biochemistry, College of Arts and Sciences, UCLA, Los Angeles, CA, 90095, USA.

Current Opinion in Chemical Biology
|February 3, 2021
PubMed
Summary

RNA binding proteins (RBPs) are crucial for RNA biology. New high-throughput screening technologies are emerging to discover small molecule probes targeting RBPs for therapeutic development.

Keywords:
Chemical probesChemoproteomicsHigh throughput screening (HTS)RNA binding proteins (RBPs)

More Related Videos

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.4K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.8K

Related Experiment Videos

Last Updated: Nov 18, 2025

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.6K
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

8.4K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • RNA binding proteins (RBPs) regulate diverse RNA biology processes.
  • Dysregulation of RBPs is linked to human diseases like cancer and neurodegenerative disorders.
  • Targeting RBPs with small molecules offers therapeutic potential but faces challenges.

Purpose of the Study:

  • To review current and emerging technologies for high-throughput screening of RBP-small molecule interactions.
  • To highlight strategies for discovering chemical probes that target individual RBPs.
  • To discuss the potential of these probes in understanding RBP function and developing new therapeutics.

Main Methods:

  • Review of existing literature on RBP-small molecule screening technologies.
  • Analysis of emerging high-throughput screening platforms.
  • Discussion of chemical probe discovery strategies for RBPs.

Main Results:

  • Identification of various high-throughput screening technologies applicable to RBP-small molecule discovery.
  • Highlighting the progress and challenges in developing chemical probes for RBPs.
  • Emphasizing the growing interest and investment in RBP-targeted therapeutics.

Conclusions:

  • High-throughput screening technologies are essential for advancing RBP chemical probe discovery.
  • Targeting RBPs holds significant promise for treating various human diseases.
  • Further development of screening strategies will unlock the therapeutic potential of RBPs.