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Pluripotential and committed hemopoietic stem cells. A hypothesis.

G Brecher

    Nouvelle Revue Francaise D'Hematologie; Blood Cells
    |January 1, 1977
    PubMed
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    Hematopoiesis regulation primarily involves committed stem cells, not quiescent pluripotential stem cells. New evidence suggests pluripotential stem cells activate with specific stimuli, challenging existing models.

    Area of Science:

    • Hematology
    • Stem Cell Biology
    • Cellular Regulation

    Background:

    • Hemopoiesis (blood cell formation) regulation is crucial for maintaining blood cell populations.
    • Current understanding suggests pluripotential stem cells (PSCs) may be actively involved in normal hematopoiesis.
    • Committed stem cell compartments are known to expand and contract during blood cell production.

    Purpose of the Study:

    • To propose and evaluate a hypothesis where hemopoiesis regulation relies on committed stem cell compartments.
    • To investigate the role of pluripotential stem cells (PSCs) in normal hematopoiesis, hypothesizing their quiescent state.
    • To present preliminary evidence supporting the hypothesis of normally quiescent PSCs.

    Main Methods:

    • Analysis of erythropoietin's effect on red cell precursors.

    Related Experiment Videos

  • Quantification of marrow mitoses in recognizable red and white cell precursors.
  • Spleen nodule assays to assess PSC compartment expansion.
  • Tritiated thymidine suicide data analysis.
  • Transfusion of normal PSCs into irradiated isologous mice.

    • Main Results:

    • Erythropoietin targets committed red cell precursors, not PSCs.
    • The vast majority of marrow mitoses occur in committed precursors, not PSCs.
    • The PSC compartment in marrow shows limited expansion capacity.
    • Tritiated thymidine data suggests some PSCs may cycle (up to 20% in certain mouse strains).
    • Transfused PSCs proliferate post-irradiation, indicating activation by specific stimuli.

    Conclusions:

    • Hemopoiesis regulation is primarily driven by the expansion/contraction of committed stem cell compartments.
    • Pluripotential stem cells (PSCs) are likely normally quiescent and not significantly involved in routine hematopoiesis.
    • Special stimuli are required to activate normally quiescent PSCs, challenging the assumption of their constant involvement or loss.
    • The hypothesis of committed stem cell regulation is supported by preliminary findings.