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Directionality of Nuclear Transport01:42

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Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
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Endogenously expressed Ranbp2 is not at the axon initial segment.

Yuki Ogawa1, Matthew N Rasband2

  • 1Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.

Journal of Cell Science
|February 4, 2021
PubMed
Summary
This summary is machine-generated.

Ranbp2 (nuclear pore complex protein) is not found at the axon initial segment (AIS). Previous studies used a non-specific antibody, leading to incorrect localization findings for Ranbp2.

Keywords:
AntibodyAxonCytoskeletonNup358Ranbp2

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Area of Science:

  • Cell Biology
  • Neuroscience
  • Molecular Biology

Background:

  • Ranbp2 (also known as Nup358) is a nucleoporin protein crucial for the nuclear pore complex.
  • Previous research suggested Ranbp2 localizes to the axon initial segment (AIS), a critical neuronal structure.

Purpose of the Study:

  • To re-evaluate the localization of Ranbp2, specifically at the AIS.
  • To determine if endogenous Ranbp2 is present at the AIS.

Main Methods:

  • Antibody specificity mapping using amino acid sequence analysis.
  • Neurofascin (Nfasc) expression silencing in primary neurons.
  • Exogenous expression of Ranbp2 fragments.
  • CRISPR/Cas9 gene editing to tag endogenous Ranbp2.

Main Results:

  • The antibody used in prior studies was found to bind to both Ranbp2 and neurofascin.
  • Silencing neurofascin abolished AIS staining by the antibody.
  • An N-terminal fragment of Ranbp2 localized to the AIS and interacted with microtubules.
  • Endogenous Ranbp2, tagged at various sites, was consistently found at the nuclear membrane, not the AIS.

Conclusions:

  • Endogenous Ranbp2 does not localize to the axon initial segment.
  • Previous reports of Ranbp2 at the AIS were likely due to antibody cross-reactivity with neurofascin.