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Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
Arthur Luhur1,2,3, Daniel Mariyappa1,2,3, Kristin M Klueg1,2,3
1Drosophila Genomics Resource Center.
Micropublication Biology
|February 4, 2021
Abstract:
We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against Rho1 in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in Drosophila S2 cells.
