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Digital Duplex Homogeneous Immunoassay by Counting Immunocomplex Labeled with Quantum Dots.

Xiaojun Liu1, Yuanyuan Sun1, Xinyi Lin1

  • 1School of Chemistry and Materials Science, Jiangsu Normal University, 101 Shanghai Road, Tongshan District, Xuzhou 221116, Jiangsu, China.

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|February 4, 2021
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Summary

This study introduces a novel spectral imaging immunoassay for detecting cancer biomarkers carcino-embryonic antigen (CEA) and α-fetoprotein (AFP). The method uses quantum dot aggregates for sensitive and accurate quantification in plasma.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Digital multiplexed homogeneous immunoassays offer high sensitivity and throughput.
  • Current methods face challenges in sensitivity and sample volume.
  • Quantum dots (QDs) present unique optical properties for bio-detection.

Purpose of the Study:

  • To develop a spectral imaging-based multiplex homogeneous immunoassay.
  • To detect cancer biomarkers carcino-embryonic antigen (CEA) and α-fetoprotein (AFP).
  • To demonstrate the accuracy and reliability of the developed method.

Main Methods:

  • Utilized quantum dot (QD) aggregates for sandwich-structured immunocomplex formation.
  • Employed spectral imaging microscopy with transmission gratings for analysis.
  • Differentiated biomarkers based on dual-color QD aggregate spectral patterns and same-color spectral blue-shifts.

Main Results:

  • Achieved limits of detection of 0.02 pM for AFP and 0.10 pM for CEA (S/N=3).
  • Successfully quantified model proteins in human plasma samples.
  • Demonstrated high sensitivity and specificity through distinct spectral signatures.

Conclusions:

  • The spectral imaging immunoassay provides a sensitive and reliable method for multiplex biomarker detection.
  • This approach offers advantages in throughput and sample consumption.
  • The QD-aggregate-based spectral imaging holds promise for clinical diagnostics.