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Fluorescence polarization system for rapid COVID-19 diagnosis
Chang Yeol Lee1, Ismail Degani2, Jiyong Cheong3
1Institute for Basic Science (IBS), Center for Nanomedicine, Seoul, South Korea; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
Biosensors & Bioelectronics
|February 4, 2021
Summary
A new portable COVID-19 test, CODA (CRISPR Optical Detection of Anisotropy), provides rapid, onsite diagnosis. This assay detects SARS-CoV-2 RNA in 20 minutes, aiding prompt containment measures.
Area of Science:
- Molecular Diagnostics
- Biotechnology
- Point-of-Care Testing
Background:
- Prompt diagnosis, isolation, and tracing are crucial for controlling COVID-19.
- Current molecular tests require central labs, causing delays in critical decisions.
- Need for rapid, accessible diagnostic tools for effective pandemic response.
Purpose of the Study:
- To develop a portable assay system for rapid, onsite COVID-19 diagnosis.
- To create a point-of-care device for timely detection of SARS-CoV-2 RNA.
- To improve diagnostic accessibility and reduce turnaround time for COVID-19 testing.
Main Methods:
- Developed CODA (CRISPR Optical Detection of Anisotropy) combining isothermal amplification and CRISPR/Cas12a activation.
- Utilized ratiometric measurement of fluorescent anisotropy for high signal-to-noise ratio detection.
- Integrated optoelectronics, heater, and microcontroller into a compact, standalone device for point-of-care operation.
Main Results:
- The CODA system detected SARS-CoV-2 RNA within 20 minutes from sample loading.
- Achieved a limit of detection as low as 3 copies/μL.
- Demonstrated accurate classification of COVID-19 status in clinical samples, aligning with gold-standard diagnostics.
Conclusions:
- The CODA system offers a rapid, accurate, and portable solution for onsite COVID-19 diagnosis.
- This technology can significantly improve diagnostic turnaround time and support public health interventions.
- CODA represents a promising advancement in point-of-care molecular diagnostics for infectious diseases.
