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Microbial physiological engineering increases the efficiency of microbial cell factories.

Hui Liu1,2,3, Yanli Qi1,2,3, Pei Zhou1,2,3

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.

Critical Reviews in Biotechnology
|February 5, 2021
PubMed
Summary

Microbial physiological engineering (MPE) enhances microbial cell factory efficiency by integrating synthetic and systems biology. This approach addresses limitations in current tools to boost chemical production rates and yields.

Keywords:
Physiological functionsgrowth performancemicrobial cell factoriesproducts transportationstress tolerancesubstrate utilization

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Systems Biology

Background:

  • Microbial cell factories are essential for chemical production.
  • Current biotechnological tools have limitations in improving cell factory efficiency.
  • Boosting microbial efficiency (titer, rate, yield) remains a significant challenge.

Purpose of the Study:

  • To propose microbial physiological engineering (MPE) as a strategy to enhance microbial cell factory efficiency.
  • To provide a new framework for overcoming bottlenecks in low-efficiency cell factories.

Main Methods:

  • Integrating tools from synthetic biology and systems biology.
  • Characterizing and regulating physiological functions within cell factories.
  • Focusing MPE strategies on substrate utilization, growth, stress tolerance, and product export.

Main Results:

  • MPE offers a novel approach to improve the efficiency of microbial cell factories.
  • The strategy addresses key physiological limitations hindering chemical synthesis.
  • A framework is provided for optimizing cell factory performance.

Conclusions:

  • Microbial physiological engineering presents a promising strategy for advancing cell factory capabilities.
  • This integrated approach can overcome existing limitations and boost production efficiency.
  • The proposed framework offers a pathway to more efficient and robust microbial chemical production.