A long-term controlled drug-delivery with anionic beta cyclodextrin complex in layer-by-layer coating for...

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Nanotechnology

Background:

• Controlled drug release is crucial for implantable devices.
• Titanium implants require effective strategies to prevent infection.
• Polymer multilayers offer versatile platforms for drug incorporation.

Purpose of the Study:

• To develop and evaluate a novel drug-delivery system for controlled tetracycline release.
• To investigate the retention and sustained release of tetracycline from poly(acrylic acid)/poly(l-lysine) multilayers.
• To assess the antibacterial efficacy and cytocompatibility of the developed system.

Main Methods:

• Fabrication of ten double layers ([PAA/PLL]10) of poly(acrylic acid) (PAA) and poly(l-lysine) (PLL) on titanium.
• Complexation of tetracycline (TC) with anionic beta cyclodextrin (β-CD) for enhanced retention.
• Layer-by-layer (LbL) assembly of drug-loaded multilayers.
• In vitro drug release studies over 30 days.
• Antibacterial assays against Staphylococcus aureus.
• Cytotoxicity assessment using human gingival fibroblasts.

Main Results:

• Demonstrated controlled drug release over 15 days and sustained release over 30 days.
• Confirmed tetracycline retention within the LbL system for 30 days via qualitative imaging.
• Established sustained antibacterial activity against Staphylococcus aureus, even after 30 days of incubation.
• Showcased non-cytotoxic effects, supporting normal human gingival fibroblast growth.

Conclusions:

• The [PAA/PLL]10/TC/anionic β-CD system effectively delivers tetracycline with sustained antibacterial properties.
• The use of anionic β-CD enhances drug incorporation and retention in LbL coatings.
• This approach shows promise for developing advanced drug-eluting coatings for implantable percutaneous devices.