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Formulation of stabilizer-free, nontoxic PLGA and elastin-PLGA nanoparticle delivery systems
Zachary R Stromberg1, M Lisa Phipps2, Harsha D Magurudeniya2
1Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos NM 87545, USA.
International Journal of Pharmaceutics
|February 5, 2021
Summary
Stabilizer-free poly(lactic-co-glycolic acid) (PLGA) nanoparticles were developed for drug and vaccine delivery. These novel nanoparticles show exceptional stability and biocompatibility, offering a safer alternative to traditional formulations.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery
Background:
- Poly(lactic-co-glycolic acid) (PLGA) nanoparticles are widely used for drug and vaccine delivery due to tunable size and sustained release.
- Toxic stabilizers like polyvinyl alcohol (PVA) limit PLGA nanoparticle utility, making stabilizer-free formulations desirable but challenging to prepare.
Purpose of the Study:
- To develop a tunable, stabilizer-free PLGA nanoparticle formulation for plasmid DNA encapsulation.
- To create a hybrid nanoparticle with elastin-like polymers for enhanced stability and biocompatibility.
Main Methods:
- Fabrication of PLGA nanoparticles using solvent evaporation methods.
- Assessment of particle size and stability under physiological conditions (PBS at 37°C).
- Modification with elastin-like polymers for increased immune tolerance.
Main Results:
- The most stable PLGA formulation (P4) featured a higher L:G ratio (65:35), lower molecular weight (MW), and a carboxyl terminus.
- P4 nanoparticles demonstrated stability comparable to PVA-stabilized nanoparticles but with significantly reduced cytotoxicity.
- Particle size was tunable via PLGA stoichiometry, maintaining DNA encapsulation efficiency.
Conclusions:
- A method for tunable, stabilizer-free PLGA nanoparticle formulation was successfully developed.
- These nanoparticles are suitable for drug and vaccine delivery applications.
- The incorporation of elastin-like polymers enhances immune tolerance and nanoparticle stability.
