Anti-bacterial and wound healing-promoting effects of zinc ferrite nanoparticles

Increasing antibiotic resistance continues to focus on research into the discovery of novel antimicrobial agents. Due to its antimicrobial and wound healing-promoting activity, metal nanoparticles have attracted attention for dermatological applications. This study is designed to investigate the scope and bactericidal potential of zinc ferrite nanoparticles (ZnFe₂O₄ NPs), and the mechanism of anti-bacterial action along with cytocompatibility, hemocompatibility, and wound healing properties.

ZnFe₂O₄ NPs were synthesized via a modified co-precipitation method. Structure, size, morphology, and elemental compositions of ZnFe₂O₄ NPs were analyzed using X-ray diffraction pattern, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. In PrestoBlue and live/dead assays, ZnFe₂O₄ NPs exhibited dose-dependent cytotoxic effects on human dermal fibroblasts. In addition, the hemocompatibility assay revealed that the NPs do not significantly rupture red blood cells up to a dose of 1000 µg/mL. Bacterial live/dead imaging and zone of inhibition analysis demonstrated that ZnFe₂O₄ NPs showed dose-dependent bactericidal activities in various strains of Gram-negative and Gram-positive bacteria. Interestingly, NPs showed antimicrobial activity through multiple mechanisms, such as cell membrane damage, protein leakage, and reactive oxygen species generation, and were more effective against gram-positive bacteria. Furthermore, in vitro scratch assay revealed that ZnFe₂O₄ NPs improved cell migration and proliferation of cells, with noticeable shrinkage of the artificial wound model.

This study indicated that ZnFe₂O₄ NPs have the potential to be used as a future antimicrobial and wound healing drug.