Electrochemiluminescence biosensor for microRNA determination based on AgNCs@MoS₂ composite with...

A novel electrochemiluminescence (ECL) biosensor was fabricated for miRNA-162a detection by using silver nanoclusters/molybdenum disulfide (AgNCs@MoS₂) as an ECL material, peroxodisulfate (S₂O₈^2-) as a co-reactant, and semicarbazide (Sem) as a co-reaction accelerator. Firstly, hairpin probe H_a modified on AgNCs@MoS₂/GCE was unfolded based on its hybridization with target microRNA. Then, the unfolded H_a can further be hybridized with another hairpin DNA of H_b on (AuNPs-semicarbazide)@Cu-MOF, resulting in the release of target microRNA, which further causes a cyclic hybridization. This creates more (AuNPs-semicarbazide)@Cu-MOF on the electrode surface, achieving cyclic hybridization signal amplification. Strikingly, due to the presence of Sem, accelerating the reduction of S₂O₈^2- and resulting in the generation of more oxidant intermediates of SO₄^2-, the amount of excited states of Agincreases to further amplify the ECL signal. The biosensor exhibited high sensitivity with a low LOD of 1.067 fM, indicating that the introduction of co-reaction accelerators can provide an effective method for signal amplification. The applicability of this method was assessed by investigating the effect of Pb(II) ion on miRNA-162a expression level in maize seedling leaves. A novel electrochemiluminescence biosensor was fabricated for miRNA-162a detection by using silver nanoclusters/molybdenum disulfide as an ECL material, peroxodisulfate as a co-reactant, and semicarbazide as a co-reaction accelerator.