Jove
Visualize
Contact Us

Related Concept Videos

Trihybrid Crosses02:27

Trihybrid Crosses

24.7K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
24.7K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

20.6K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
20.6K
Transgenic Plants02:50

Transgenic Plants

8.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.0K
Monohybrid Crosses01:20

Monohybrid Crosses

236.2K
Overview
236.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An <i>Agrobacterium tumefaciens EHA105</i>-Based GA<i>A</i>NTRY Recipient Strain Generates High-Quality Transgenic <i>Arabidopsis</i> and Potato.

Microorganisms·2026
Same author

Introducing Pattern Recognition Receptors in Potato Confers Enhanced Resistance to Ralstonia solanacearum but Not a Vector Borne Pathogen.

Plant biotechnology journal·2025
Same author

Integrative genome and transcriptome analysis identifies smoke-responsive glycosyltransferases in grapevine berries.

Journal of experimental botany·2025
Same author

Development of an improved construct for spectinomycin selection in plant transformation.

BMC research notes·2025
Same author

Novel <i>Agrobacterium fabrum</i> str. 1D1416 for Citrus Transformation.

Microorganisms·2024
Same author

<i>Agrobacterium</i>-Mediated Transformation of the Dwarf Soybean MiniMax.

Plants (Basel, Switzerland)·2024
Same journal

Genes, Putative Long-Lived mRNAs and Pathways Underlying Genotypic Differences in Rice Seed Storability and Seed Dormancy.

Rice (New York, N.Y.)·2026
Same journal

Correction: Developmental Dynamics of Intercalary Meristem and Pith Cavity in Rice Stems.

Rice (New York, N.Y.)·2026
Same journal

Structural Volume Composition of Internodes is a Key Morphological Factor Contributing to Culm Non-structural Carbohydrate Accumulation in Rice.

Rice (New York, N.Y.)·2026
Same journal

Natural Variation and Expression of OsCFF1 Affect the Formation of Rice Chalkiness.

Rice (New York, N.Y.)·2026
Same journal

OsWRKY11 Regulates Rice Leaf Angle Via Transcriptional Activation of OsGSR1.

Rice (New York, N.Y.)·2026
Same journal

CRISPR/Cas9-Mediated Mutagenesis of OsERF94 Enhances Pre-Harvest Sprouting in Rice.

Rice (New York, N.Y.)·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 18, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K

Efficient Gene Stacking in Rice Using the GAANTRY System.

Leyla T Hathwaik1, James Horstman1, James G Thomson1

  • 1United States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research Unit, Albany, CA, 94710, USA.

Rice (New York, N.Y.)
|February 6, 2021
PubMed
Summary
This summary is machine-generated.

The Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY (GAANTRY) system efficiently stacks multiple genes in rice, enabling improved crop traits. This genetic engineering tool facilitates the development of high-quality transgenic rice lines.

Keywords:
AgrobacteriumGene stackingGenetic engineeringOryza sativaSite-specific recombinase

More Related Videos

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

12.9K
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

6.7K

Related Experiment Videos

Last Updated: Nov 18, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.9K
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

12.9K
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

6.7K

Area of Science:

  • Plant biotechnology
  • Crop genetics
  • Molecular biology

Background:

  • Genetic engineering offers a powerful approach to enhance rice grain quality and yield.
  • Conventional breeding methods face limitations in introducing multiple desirable traits simultaneously.
  • The GAANTRY system provides a precise method for assembling and transferring multiple genes into plant genomes.

Purpose of the Study:

  • To evaluate the efficacy of the GAANTRY system for transgene stacking in rice, a monocotyledonous crop.
  • To assess the ability of GAANTRY to stably integrate and express multiple genes in rice.
  • To determine the efficiency of generating high-quality transgenic rice events using GAANTRY.

Main Methods:

  • Assembly of two new Transfer-DNA (T-DNA) constructs with five (16.9 kb) and eleven (37.4 kb) cargo sequences using the GAANTRY system.
  • Transformation of these constructs into rice plants.
  • Characterization of 53 independent transgenic rice events to assess transgene integration, expression, and quality.

Main Results:

  • Over 50% of transgenic rice events successfully integrated all desired cargo sequences and exhibited the introduced traits.
  • More than 18% of the analyzed lines were high-quality events, featuring a single copy of the transgenes and lacking extraneous DNA.
  • The GAANTRY system demonstrated robustness in assembling and transferring large DNA payloads into rice.

Conclusions:

  • The GAANTRY system is a precise, versatile, and effective tool for transgene stacking in rice.
  • This technology has significant potential for accelerating the genetic improvement of rice and other cereal crops.
  • GAANTRY simplifies the process of introducing complex genetic modifications into important crop species.